| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.233952 |
| Chromosome: | chromosome 13 |
| Location: | 2120746 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g577800 | (1 of 1) PTHR11089:SF24 - GUANINE NUCLEOTIDE-BINDING PROTEIN-LIKE 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCACCCCGGCCGCACCAAGCACTACCAG |
| Internal bar code: | GCGCAGGGCGGGTCGCAGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 428 |
| LEAP-Seq percent confirming: | 99.661 |
| LEAP-Seq n confirming: | 294 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGTGTGCCATGTCCTCGT |
| Suggested primer 2: | GTGTTGAAGAGCACAAGGCA |