Insertion junction: LMJ.RY0402.234002_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.234002
Chromosome:chromosome 12
Location:3779831
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g515250 ROC7 Rythm Of Chloroplast bioluminescence 7; (1 of 1) PF09758 - Uncharacterised conserved protein (FPL) CDS/intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCAGCTCCACCTCCTGCCCCTCCATGAC
Internal bar code:TTTAGCGGCTCACAAGGATCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:536
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GTGATCACAGTTCCGGTGTG
Suggested primer 2:TGCTAGGCTGGACCTTCAGT