| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.234007 |
| Chromosome: | chromosome 9 |
| Location: | 1644578 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397100 | (1 of 39) IPR009003 - Peptidase S1, PA clan | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGCCTCCGGCCGCGCCTTGCTTCGCATC |
| Internal bar code: | GAAACGCGAACGGACTAGGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 549 |
| LEAP-Seq percent confirming: | 99.3036 |
| LEAP-Seq n confirming: | 713 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGCGAAGCTCTTGACGTT |
| Suggested primer 2: | GCGCCTAGACAGTCCATAGC |