| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.234010 |
| Chromosome: | chromosome 14 |
| Location: | 1241642 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g616400 | (1 of 8) PF00011 - Hsp20/alpha crystallin family (HSP20) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGAACAAGGTGCACGGGCAACACAGCAG |
| Internal bar code: | CAGGCACTATGCGTAAGTTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 506 |
| LEAP-Seq percent confirming: | 97.2064 |
| LEAP-Seq n confirming: | 3410 |
| LEAP-Seq n nonconfirming: | 98 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCACCTCCATCATCATC |
| Suggested primer 2: | GCACATCAATAACGACACGC |