| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.234025 |
| Chromosome: | chromosome_6 |
| Location: | 1736245 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g261750 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGGTCAGCTGTGCATTCCCCTGGAGAGAAG |
| Internal bar code: | GGGAAAAGGAGCTTAGTCAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 435 |
| LEAP-Seq percent confirming: | 92.757 |
| LEAP-Seq n confirming: | 397 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTCCAAGCTGACCAAGAT |
| Suggested primer 2: | GGTCAGGCATCAGGTACGTT |