| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.234044 |
| Chromosome: | chromosome 3 |
| Location: | 6212081 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g192450 | CYA3 | Adenylate cyclase; (1 of 70) 4.6.1.1 - Adenylate cyclase / ATP pyrophosphate-lyase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAAGCGGGCTGCGGAGCCACACAGTGAAC |
| Internal bar code: | GACGGTGCTAACAGAGTCAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 655 |
| LEAP-Seq percent confirming: | 98.7887 |
| LEAP-Seq n confirming: | 2202 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGCCTTCAAGAGCGACAG |
| Suggested primer 2: | TGGCATGAGAATGTTGGGTA |