| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.234047 |
| Chromosome: | chromosome 12 |
| Location: | 8423113 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g548500 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGGCTGTCAAGGGGCCCAACGATGGGCC |
| Internal bar code: | GTTAATCAGGCTGACGGGGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 579 |
| LEAP-Seq percent confirming: | 98.8636 |
| LEAP-Seq n confirming: | 435 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGACTGTGTGCAATGGACC |
| Suggested primer 2: | GCGTCCAGAGACTGTGACAA |