| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.234057 |
| Chromosome: | chromosome 12 |
| Location: | 1681588 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g485850 | CPLD64 | Conserved in the Plant Lineage and Diatoms; (1 of 2) PF06916 - Protein of unknown function (DUF1279) (DUF1279) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCTGAGCTTGCCCTCACCGCCCTCGTCC |
| Internal bar code: | CTTTGCAGGCAGGTTAGTCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1172 |
| LEAP-Seq percent confirming: | 98.5932 |
| LEAP-Seq n confirming: | 2593 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTGGTCAACATGCTGTGT |
| Suggested primer 2: | CAGCTCCCACTCACCTTCTC |