| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.234069 |
| Chromosome: | chromosome 12 |
| Location: | 2460830 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g506851 | BBS3A | (1 of 2) K07951 - ADP-ribosylation factor-like protein 6 (ARL6, BBS3); Bardet-Biedl syndrome-3 associated protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACCTGCGTTTCATCCGCCCACCCACCTC |
| Internal bar code: | ACGACGTGCTTGGGTCAGGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 346 |
| LEAP-Seq percent confirming: | 70.3297 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTATGCTGGTTGTTGGGT |
| Suggested primer 2: | CTTCCCAGTCCCTTAGACCC |