| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.234072 |
| Chromosome: | chromosome 12 |
| Location: | 7226391 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g558352 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGGTTGAAGCGCCGCACGTGCGCTCGTC |
| Internal bar code: | GTCGTTGACAGGTCGGTCGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 575 |
| LEAP-Seq percent confirming: | 95.5928 |
| LEAP-Seq n confirming: | 1540 |
| LEAP-Seq n nonconfirming: | 71 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCATCAGCAACAAGTGTCT |
| Suggested primer 2: | GGGTAGGAAACAAAGTGCCA |