Insertion junction: LMJ.RY0402.234079_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.234079
Chromosome:chromosome 17
Location:6208449
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g741950 BBS1 Bardet-Biedl syndrome-1 associated protein; (1 of 1) K16746 - Bardet-Biedl syndrome 1 protein (BBS1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAACAAATCGCTTCCATACACAAGAACT
Internal bar code:GAGTAGACCGCGGCGTACAGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:637
LEAP-Seq percent confirming:98.9385
LEAP-Seq n confirming:3542
LEAP-Seq n nonconfirming:38
LEAP-Seq n unique pos:67

Suggested primers for confirmation by PCR

Suggested primer 1:GTCGTTTTGTCCCACTCGAT
Suggested primer 2:CACCAGGTGATCGTTGTCAC