Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.234079 |
Chromosome: | chromosome_17 |
Location: | 6208449 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g741950 | BBS1 | Bardet-Biedl syndrome-1 associated protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCCAACAAATCGCTTCCATACACAAGAACT |
Internal bar code: | GAGTAGACCGCGGCGTACAGAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 637 |
LEAP-Seq percent confirming: | 98.9385 |
LEAP-Seq n confirming: | 3542 |
LEAP-Seq n nonconfirming: | 38 |
LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTCGTTTTGTCCCACTCGAT |
Suggested primer 2: | CACCAGGTGATCGTTGTCAC |