| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.234082 |
| Chromosome: | chromosome 10 |
| Location: | 5043210 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g455700 | PAP5 | Non-canonical poly(A) polymerase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTGATCAAGAACAAGCGAGGTTGTAGCC |
| Internal bar code: | ATGGCACCCCGCGCAAAAGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 278 |
| LEAP-Seq percent confirming: | 93.8462 |
| LEAP-Seq n confirming: | 305 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGAGGCAGCGAGGTACAC |
| Suggested primer 2: | CAATAACCGCGTTTCAACCT |