| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.234085 |
| Chromosome: | chromosome 2 |
| Location: | 1675061 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g085750 | (1 of 8) PF13416 - Bacterial extracellular solute-binding protein (SBP_bac_8) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCACACTACCGCCAGCGTCAGCTGGCCA |
| Internal bar code: | CGATCCCACACGGGTCTAGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 626 |
| LEAP-Seq percent confirming: | 99.1679 |
| LEAP-Seq n confirming: | 8223 |
| LEAP-Seq n nonconfirming: | 69 |
| LEAP-Seq n unique pos: | 81 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCGTAAACCATCGCTTTG |
| Suggested primer 2: | TAGACTGCTTCCGTCCGAGT |