| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.234112 |
| Chromosome: | chromosome 7 |
| Location: | 2352080 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g328226 | (1 of 6) IPR000817 - Prion protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGCCCGGCCTCGTTCTTGGTTTCTGCCC |
| Internal bar code: | TGAACACGAGAAGTGCTGATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 480 |
| LEAP-Seq percent confirming: | 98.6813 |
| LEAP-Seq n confirming: | 1347 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGGCTGGTATCTTCACC |
| Suggested primer 2: | CGGCAACAGCAGCAGTAGTA |