Insertion junction: LMJ.RY0402.234163_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.234163
Chromosome:chromosome 14
Location:3824907
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g632700 (1 of 9) 2.7.10.1 - Receptor protein-tyrosine kinase / Receptor protein tyrosine kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGACGTAACCCACCGTATTCGCTACGTTCA
Internal bar code:TTCGCCCGACTTTCGCGGGAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:294
LEAP-Seq percent confirming:99.1159
LEAP-Seq n confirming:10875
LEAP-Seq n nonconfirming:97
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TTGTCTGCACTTCCGTTGAG
Suggested primer 2:CAGACGCATTAGTGAGGCAA