Insertion junction: LMJ.RY0402.234217_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.234217
Chromosome:chromosome 16
Location:1308188
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g651600 (1 of 274) IPR020683 - Ankyrin repeat-containing domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCTTGTCGAACCGGGGAGACCGCGCTCCC
Internal bar code:GTAGGGGGTTATGTGTCAGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:473
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:127
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GGGGCTTCTGTCGTATGTGT
Suggested primer 2:AGTTTCCTCCAACACATGCC