| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.234219 |
| Chromosome: | chromosome 3 |
| Location: | 5052866 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g180850 | SEC23A | (1 of 1) K14006 - protein transport protein SEC23 (SEC23); COP-II coat subunit | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTACACTGTACTTTTGAGTGCCTGAAGT |
| Internal bar code: | CACGATCCGGCAAAAATTGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 62 |
| LEAP-Seq percent confirming: | 87.0968 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGGTATGTGGCCTTGTTT |
| Suggested primer 2: | GTCTCGGTGGTACAATGGCT |