| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.234220 |
| Chromosome: | chromosome_10 |
| Location: | 443751 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g420800 | FAP46 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCAGCGGCCGGTGCAGGCTGCGGCTCTTGA |
| Internal bar code: | GGCTAAGCAGTGCGGTAGAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 510 |
| LEAP-Seq percent confirming: | 98.1092 |
| LEAP-Seq n confirming: | 467 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTCATGTGTGCTCAGGGA |
| Suggested primer 2: | TGCATTTCTGTCGTAGCCTG |