| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.234220 |
| Chromosome: | chromosome 12 |
| Location: | 4334288 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g520100 | CYG66 | (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGACTCGGCAGCCACGGCCGGTCTAGACA |
| Internal bar code: | ACCGTCTCATCTCGGTGCCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 661 |
| LEAP-Seq percent confirming: | 84.2736 |
| LEAP-Seq n confirming: | 5273 |
| LEAP-Seq n nonconfirming: | 984 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACCGCACTGGGTATGACA |
| Suggested primer 2: | TCTAACCAGGATGGCGAAAC |