Insertion junction: LMJ.RY0402.234221_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.234221
Chromosome:chromosome 11
Location:1102222
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g467678 COP6,HKR2 (1 of 5) IPR001660//IPR013761 - Sterile alpha motif domain // Sterile alpha motif/pointed domain; Histidine cyclase rhodopsin 2 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGCCCCTTCTCCTCCCCTGCCGCCCCCCT
Internal bar code:CGCTGACGGCAAGACTGTCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:904
LEAP-Seq percent confirming:95.3972
LEAP-Seq n confirming:1285
LEAP-Seq n nonconfirming:62
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:CCGGTCAGGTTGTACAGGAT
Suggested primer 2:AGAGCGAGGAAGGGAAGAAG