Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.234235 |
Chromosome: | chromosome 8 |
Location: | 544383 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre08.g359300 | PHO1 | (1 of 1) PTHR11575//PTHR11575:SF29 - 5'-NUCLEOTIDASE-RELATED // SUBFAMILY NOT NAMED; Alkaline phosphatase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGTGGGCCATCCTGGACGCGGGTAACGG |
Internal bar code: | CCTTTCATGCGGGTCCGACGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 485 |
LEAP-Seq percent confirming: | 99.4152 |
LEAP-Seq n confirming: | 170 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACTTCGTGTCAAACCCAACC |
Suggested primer 2: | CTCCCCTGGTTGCACTGTAT |