Insertion junction: LMJ.RY0402.234238_4

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.234238
Chromosome:chromosome 9
Location:2203934
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g393000 CSR6 (1 of 6) 2.8.2.8 - [Heparan sulfate]-glucosamine N-sulfotransferase / PAPS:N-desulfoheparin sulfotransferase; Carbohydrate sulfotransferase-related 6 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGATCCAACCATGGCGCCCGGGCGACGTGA
Internal bar code:GCGCAAATGTCATCTGGGCATT

Confirmation - LEAP-Seq

LEAP-Seq distance:415
LEAP-Seq percent confirming:99.1761
LEAP-Seq n confirming:1926
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GTATTCAAAGGGGGCATGTG
Suggested primer 2:CTTTGCTGTACGCTATGCCA