| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.234240 |
| Chromosome: | chromosome 9 |
| Location: | 7653181 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g415300 | GT90-19,GT90F19 | GT90 family protein 19; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCGTAGCCTACCGTGCTGGGCCGTGGGG |
| Internal bar code: | TCGTGGGTATGGCCGTCGTATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1225 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGAGGTGTCGCTGAAAA |
| Suggested primer 2: | TCTTCTAGCCGCATCCTGTT |