| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.234244 |
| Chromosome: | chromosome 14 |
| Location: | 2185065 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g622550 | (1 of 1) PTHR10953//PTHR10953:SF145 - UBIQUITIN-ACTIVATING ENZYME E1 // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCCAGGAGTTCCTGGTGCCGGACAGCGC |
| Internal bar code: | GGTGGACTGATGTGGCGGCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 902 |
| LEAP-Seq percent confirming: | 90.3606 |
| LEAP-Seq n confirming: | 19723 |
| LEAP-Seq n nonconfirming: | 2104 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAGACAAAGCAGAAGCCC |
| Suggested primer 2: | TCCCATCACTTCCTGTGTCA |