| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.234275 |
| Chromosome: | chromosome 12 |
| Location: | 545461 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g492850 | POC19 | Proteome of centriole protein 19; (1 of 1) PF10595 - Uncharacterised protein family UPF0564 (UPF0564) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAGAGCCCTGCCATGGTGCATCGGTTAC |
| Internal bar code: | GATCTACATTTTCACTCGGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 666 |
| LEAP-Seq percent confirming: | 99.0978 |
| LEAP-Seq n confirming: | 27461 |
| LEAP-Seq n nonconfirming: | 250 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCGTAGCTGATTTGCGTG |
| Suggested primer 2: | CATTCCGGTATGTTGTGCTG |