Insertion junction: LMJ.RY0402.234302_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.234302
Chromosome:chromosome 17
Location:49141
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g696450 (1 of 19) IPR000225//IPR016024 - Armadillo // Armadillo-type fold 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTACAGCTCGACGCTCGTCGTGTCCTACGC
Internal bar code:GCGGAATACAACCTCGCGGACG

Confirmation - LEAP-Seq

LEAP-Seq distance:1336
LEAP-Seq percent confirming:99.4318
LEAP-Seq n confirming:12950
LEAP-Seq n nonconfirming:74
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:AAAGCCAAATGCACCAAATC
Suggested primer 2:ATTCATTCTAGCTGCGCGAT