Insertion junction: LMJ.RY0402.234337_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.234337
Chromosome:chromosome 3
Location:1284618
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g150251 (1 of 80) IPR003882 - Pistil-specific extensin-like protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTTTTACTTCCTGCGTGGCGACGACCCAT
Internal bar code:CTTTGGCTTCTAATGGGAAATA

Confirmation - LEAP-Seq

LEAP-Seq distance:224
LEAP-Seq percent confirming:96.89
LEAP-Seq n confirming:3022
LEAP-Seq n nonconfirming:97
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:TTTGGTTTAGTTTGGGCTGG
Suggested primer 2:CTTCACCTGTACCTCCCGTC