Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.234378 |
Chromosome: | chromosome_17 |
Location: | 2701497 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g717950 | antisense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGCAGGCACAGTAACGCACAACGCCGTTGC |
Internal bar code: | ATGGGAATATGCACGTCAATTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 515 |
LEAP-Seq percent confirming: | 99.4475 |
LEAP-Seq n confirming: | 180 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTCCTGGTAACCCCTCAACA |
Suggested primer 2: | CGAGGTACCACGGAAGTCAT |