Insertion junction: LMJ.RY0402.234385_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.234385
Chromosome:chromosome 13
Location:3734569
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre13.g589350 FBB6 Flagellar/basal body protein 6; (1 of 1) IPR000679//IPR002035//IPR013694 - Zinc finger, GATA-type // von Willebrand factor, type A // VIT domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTACAAGAGCGATGCGGAGTGCTCAGCAGT
Internal bar code:GAATCGTCACGACAATGAGCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:129
LEAP-Seq percent confirming:69.6429
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGACGGAGTTAGTGCCTC
Suggested primer 2:GAGGTAGCTGAACTCCACCG