| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.234389 |
| Chromosome: | chromosome 7 |
| Location: | 4980418 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g346800 | FAO8 | FAD-dependent oxidoreductase; (1 of 1) PTHR13847//PTHR13847:SF204 - FAD NAD BINDING OXIDOREDUCTASES // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGAGTCAGTATGGGGCCACAGGCTACTG |
| Internal bar code: | CCGGCTTCCGAGGTTGGCATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 611 |
| LEAP-Seq percent confirming: | 99.2825 |
| LEAP-Seq n confirming: | 2214 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACCACCACTACCACACAC |
| Suggested primer 2: | CCGGGAACTGGGTATGTATG |