| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.234416 |
| Chromosome: | chromosome 1 |
| Location: | 1694678 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g008950 | (1 of 1) IPR024930 - Skp domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCATATGGTACCTATGTAGGTGTAGGAAC |
| Internal bar code: | GACAAACCGTTTTCAACTGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 594 |
| LEAP-Seq percent confirming: | 99.0551 |
| LEAP-Seq n confirming: | 6185 |
| LEAP-Seq n nonconfirming: | 59 |
| LEAP-Seq n unique pos: | 127 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAGCTCATGGAGACCCTC |
| Suggested primer 2: | TCGTCAAGCAGTCGTTTGTC |