Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.234429 |
Chromosome: | chromosome_1 |
Location: | 2646796 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g015451 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATAGCGCTAGCCCCCCGCCATCCACACACA |
Internal bar code: | GGCTTGTATGGCTAGTGTGCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 210 |
LEAP-Seq percent confirming: | 99.6832 |
LEAP-Seq n confirming: | 6293 |
LEAP-Seq n nonconfirming: | 20 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATCACTGCTGCTACTGCTGC |
Suggested primer 2: | TCTCGACATCGTGAAACTGC |