| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.234492 |
| Chromosome: | chromosome 1 |
| Location: | 1742382 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g009325 | (1 of 2) 1.14.11.18 - Phytanoyl-CoA dioxygenase / Phytanoyl-CoA hydroxylase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATACGCTCCGCTCGCCTCCAGAACGTGCTC |
| Internal bar code: | GTAACGTGGGGCGAGTGACACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 685 |
| LEAP-Seq percent confirming: | 97.1429 |
| LEAP-Seq n confirming: | 68 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTGTTACCAGCGACCAGT |
| Suggested primer 2: | GTATTGCTAACCGACCCAGC |