Insertion junction: LMJ.RY0402.234499_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.234499
Chromosome:chromosome 2
Location:6817892
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g119050 CER1 Carboxyesterase-related protein; (1 of 2) K15889 - prenylcysteine alpha-carboxyl methylesterase (PCME) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAGTGGTCATCTACATAACAGGCAGGTG
Internal bar code:GGTATCCGTGCCCGAAAAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:296
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CATAATTCGGTCCAGCAGGT
Suggested primer 2:GTCGTACATTTGCGGGAGTT