| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.234520 |
| Chromosome: | chromosome 6 |
| Location: | 834478 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g254400 | FUM1 | (1 of 1) 5.3.2.2 - Oxaloacetate tautomerase / Oxaloacetate keto--enol tautomerase; Fumarate hydratase 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCACACGCCATGGCCTATACCTGCACTTC |
| Internal bar code: | GAATTCAACAATGTTTAGTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 446 |
| LEAP-Seq percent confirming: | 20.8269 |
| LEAP-Seq n confirming: | 403 |
| LEAP-Seq n nonconfirming: | 1532 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGACTACTCCCTCCACCC |
| Suggested primer 2: | GAGGAACTGGAGTGGCAGAG |