| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.234520 |
| Chromosome: | chromosome 6 |
| Location: | 834480 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g254400 | FUM1 | (1 of 1) 5.3.2.2 - Oxaloacetate tautomerase / Oxaloacetate keto--enol tautomerase; Fumarate hydratase 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGAGGCAACAGCTAGCGTGGTGCTGGA |
| Internal bar code: | GCCTGGAGTACGGGTCGTTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 858 |
| LEAP-Seq percent confirming: | 98.8757 |
| LEAP-Seq n confirming: | 3254 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGAACTGGAGTGGCAGAG |
| Suggested primer 2: | TGTGACTACTCCCTCCACCC |