| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.234530 |
| Chromosome: | chromosome 17 |
| Location: | 1766626 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g709250 | (1 of 1) PTHR23137:SF3 - VESICLE TRANSPORT PROTEIN SFT2C | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGCGGCTGGGCGTGTGCAAGGCTGGTTT |
| Internal bar code: | GATAGCGTTCTTGCTAGGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 992 |
| LEAP-Seq percent confirming: | 99.1667 |
| LEAP-Seq n confirming: | 18565 |
| LEAP-Seq n nonconfirming: | 156 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAGGGAGTACGGGTTGTGC |
| Suggested primer 2: | TGTCCAATCAAACAGGTCCA |