| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.234568 |
| Chromosome: | chromosome 7 |
| Location: | 1719491 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325711 | (1 of 2) K19363 - lipopolysaccharide-induced tumor necrosis factor-alpha factor (LITAF) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTATGAGTTAACACCGAATGTCAAGCCCCA |
| Internal bar code: | GCTTTCGTTACGAGTAGGAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 598 |
| LEAP-Seq percent confirming: | 96.9231 |
| LEAP-Seq n confirming: | 756 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGCCAATGCTTACCGTAT |
| Suggested primer 2: | CATGCTACAGTCTCCCAGCA |