| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.234577 |
| Chromosome: | chromosome 2 |
| Location: | 4943224 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g103784 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTACTCTAATGCACACAGCTACGACACAC |
| Internal bar code: | AAGAGAGCATCCGCACGTGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 802 |
| LEAP-Seq percent confirming: | 98.9887 |
| LEAP-Seq n confirming: | 2447 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAATGTCGCTCATACGCTGC |
| Suggested primer 2: | ACAGACGGGGATAGTATGCG |