| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.234599 |
| Chromosome: | chromosome 7 |
| Location: | 1543215 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g324200 | BTA1 | (1 of 1) K13621 - betaine lipid synthase (BTA1); Diacylglyceryl-N,N%252CN-trimethylhomoserine synthesis protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGGTCATCTCGTCAAGTACCGGTACTGA |
| Internal bar code: | CGGGTCTTCAAACGTCGGAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 674 |
| LEAP-Seq percent confirming: | 99.6521 |
| LEAP-Seq n confirming: | 5729 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 92 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAAGCACAACGGCAGATA |
| Suggested primer 2: | CTCAGCAGTGCAGTTGCTTC |