| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.234608 |
| Chromosome: | chromosome_2 |
| Location: | 7235244 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g145850 | FAP112 | Flagellar Associated Protein | sense | 5'UTR_intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCTTCGATGGCGGTCTACCGCTGAAAGCAA |
| Internal bar code: | CTTTCGCGTGTATCGTTCACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 612 |
| LEAP-Seq percent confirming: | 97.5138 |
| LEAP-Seq n confirming: | 1765 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTGCCATCACATACCTGG |
| Suggested primer 2: | CTCTTTCTTTTCGGGCACAG |