| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.234628 |
| Chromosome: | chromosome 7 |
| Location: | 3715988 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g337700 | PDC1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTCCGACAGCGCCAGCGCCAACAGTGTG |
| Internal bar code: | TGCGACTACATTAATTCAATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 105 |
| LEAP-Seq percent confirming: | 74.9436 |
| LEAP-Seq n confirming: | 332 |
| LEAP-Seq n nonconfirming: | 111 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCACCACGACCTCTACCG |
| Suggested primer 2: | CACACCCATGTCGTGACC |