| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.234629 |
| Chromosome: | chromosome 6 |
| Location: | 7853900 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g303000 | (1 of 24) IPR006553 - Leucine-rich repeat, cysteine-containing subtype | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTCCGCGCTCTGTGCACTGGCTCTGGG |
| Internal bar code: | CATATATGGTCGTCGTACATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 69 |
| LEAP-Seq percent confirming: | 91.5058 |
| LEAP-Seq n confirming: | 237 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGTTGTTACGGCTGGTTT |
| Suggested primer 2: | CACTAACGTGAGTCGCTCCA |