| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.234632 |
| Chromosome: | chromosome 17 |
| Location: | 1981914 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g711050 | (1 of 1) K15456 - protein KTI12 (KTI12) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTTTCAATGAGAAGAACACGCGCGCCAA |
| Internal bar code: | TCTGCCGTGGGCCACGCACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 632 |
| LEAP-Seq percent confirming: | 82.9764 |
| LEAP-Seq n confirming: | 775 |
| LEAP-Seq n nonconfirming: | 159 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCACGTTGGCAGCTAAACT |
| Suggested primer 2: | CCCCACTGCTTTGTCCTTTA |