| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.234647 |
| Chromosome: | chromosome 9 |
| Location: | 4333495 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394954 | (1 of 1) K03975 - membrane-associated protein (dedA) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTACCCATGCCCGACCCTTCACCTTGCT |
| Internal bar code: | CTACTGCGGGCATCGTTACCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 810 |
| LEAP-Seq percent confirming: | 62.3022 |
| LEAP-Seq n confirming: | 8622 |
| LEAP-Seq n nonconfirming: | 5217 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTCAACACAACTGCCTGC |
| Suggested primer 2: | ATCTGGTGTGCTGGAGGAAG |