Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.234650 |
Chromosome: | chromosome 2 |
Location: | 7902742 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAGGCGCGCAGGACACACGGACGGCCTC |
Internal bar code: | ACGGGCCTTGGGGCGCGCCCCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 626 |
LEAP-Seq percent confirming: | 73.5099 |
LEAP-Seq n confirming: | 3774 |
LEAP-Seq n nonconfirming: | 1360 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTTGCTGACAGGCATAGCAC |
Suggested primer 2: | TATCTCCAGTTGCTTGCGTG |