Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.234650 |
Chromosome: | chromosome 3 |
Location: | 267992 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g144484 | CDPK10 | Calcium/calmodulin-dependent protein kinase; (1 of 4) PTHR24349//PTHR24349:SF121 - SERINE/THREONINE-PROTEIN KINASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTCACGTGCCGGCGAAGGCGCGTGCCGG |
Internal bar code: | CCAGCACGTCTCGCTTACGGAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1132 |
LEAP-Seq percent confirming: | 99.55 |
LEAP-Seq n confirming: | 12167 |
LEAP-Seq n nonconfirming: | 55 |
LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTGCCTTCAAGTGTGTGAGC |
Suggested primer 2: | GCTGAACTACGGTACCCCAA |