| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.234658 |
| Chromosome: | chromosome 2 |
| Location: | 838498 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g079004 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATTTTACTGCTTGGCCAGTGTGAGTCCTG |
| Internal bar code: | CATACGCAGATAACCGCGTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 613 |
| LEAP-Seq percent confirming: | 98.5188 |
| LEAP-Seq n confirming: | 3126 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTTGTCTCTTCTTGAGGG |
| Suggested primer 2: | GCCACAACTGCTAGCAACAA |