| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.234668 |
| Chromosome: | chromosome 4 |
| Location: | 1914863 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g218100 | FAP25 | (1 of 1) PF11539 - Protein of unknown function (DUF3228) (DUF3228); Flagellar Associated Protein 25 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATCGGAAGAGATAACCCCTCAGCCCACG |
| Internal bar code: | CCAGGGGCATTTCGGTCTGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 80 |
| LEAP-Seq percent confirming: | 97.3756 |
| LEAP-Seq n confirming: | 1076 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAAACGCATATGGGGCCTA |
| Suggested primer 2: | CAACACCTGTCGTGGTCAAC |