Insertion junction: LMJ.RY0402.234672_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.234672
Chromosome:chromosome 16
Location:4166306
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre16.g668950 CPZ (1 of 1) 3.4.17.1 - Carboxypeptidase A / Carboxypolypeptidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACATCCACATCCACGTCCTCCACATCCA
Internal bar code:CGCCTAGCGTGTCGCTTCTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:55
LEAP-Seq percent confirming:99.1667
LEAP-Seq n confirming:357
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CCACGTCAACTATGGCAATG
Suggested primer 2:CAGACGGGACTCGAACTCAC